1,25 (OH)2 Vitamin D ELISA
This new ELISA (Enzyme-Linked Immunosorbent Assay) for 1,25(OH)2 Vitamin D is claimed to be the fastest 1,25(OH)2 Vitamin D ELISA on the market, with proven extraction method and limited number of incubation steps. It has breakable wells that offers flexibility for small and medium-sized laboratories. Performance of the assay is excellent, showing a good correlation with the Golden Standard LC-MS/MS and a cross-reactivity with the D2 form of 1,25(OH)2 Vitamin D close to 100%. Measurement of 1,25(OH)2 Vitamin D is less obvious than 25OH Vitamin D, because of its low concentration and short half-life. However, because of its metabolic activity and especially in cases of chronic renal insufficiency, it is more and more recommended to add the measurement of 1,25(OH)2 Vitamin D to the current existing 25OH Vitamin D test.
