Combination cell/microbead autoantibody IFT assay
The novel CytoBead technology offers for the first time the combination of autoantibody analysis by cell-based screening and the confirmation of the corresponding autoantigen reactivities by multiplexing, using the immunofluorescence technique (IFT) in one reaction environment. The CytoBead ANCA for example allows the screening of antineutrophil cytoplamsic antibodies (ANCA) on ethanol-fixed neutrophiles and the confirmation thereof using proteinase 3 (PR3) and myeloperoxidase (MPO) coated microbeads. Furthermore, the detection of Goodpasture antibodies, which can occur in 10% of rapid progressive glomerulonephritis patients, is integrated by adding glomerular basement membrane (GBM) coated microbeads. Additionally, this technology provides an ideal reaction environment for multiplexing antinuclear antibody (ANA) assessment and the detection of celiac disease specific antibodies. This assay format can be interpreted with a standard fluorescence microscope for semi-quantitative analysis and with the automated interpretation system Aklides for quantitative testing. CytoBead technology is an attractive alternative to classical, time-consuming single parameter antibody detection and is applicable for high throughput routine diagnostics.