Complementary qPCR diagnostic tests for sexually transmitted infection
A CE-marked multiplex real-time PCR reagent targeting Mycoplasma genitalium and Trichomonas vaginalis is adapted to the most used qPCR automated systems usually dedicated to C. trachomatis and N. gonorrhoeae. This new kit can dramatically improve the diagnosis process for patients with sexually transmitted infection. The mg219 gene was found to be in all strains of M. genitalium and was highly conserved; it is coupled to the mgPa gene and T. vaginalis. A quick and accurate detection of pathogens causing various STIs can solve a lot of problems with regard to partner contamination and supports adapted antibiotherapy regimens. Molecular diagnostics enables an easier and more reliable diagnosis than traditional methods. Mycoplasma genitalium has been convincingly linked to non-gonococcal urethritis (NGU) and nonchlamydial-non-gonococcal urethritis (NCNGU); association of M. genitalium infection and pelvic inflammatory disease (PID) aft er termination of pregnancy is an independent and strong risk factor for both cervicitis and PID. Therefore, M. genitalium infection in women is associated with clinical manifestations and complications that in some instances are amenable to prevention and treatment.