Discriminating antibody panels for rare event detection
The DuraClone RE* antibody panels provide tools to study low frequency populations of either abnormal CD5+ B cells (RE CLB), abnormal plasma cells (RE PC) or abnormal B progenitor cells (RE ALB). The three new standardized, pre-formulated DuraClone RE* kits deliver high sensitivity for rare cell populations, able to accommodate either large sample volumes or high cellular concentrations. Using dry, unitized antibody combinations helps preserve the strict assay conditions essential for reliable, reproducible rare event analysis. The DuraClone family of dry reagents simplifies sample preparation and handling. The panels eliminate errors from manual antibody pipetting, streamline workflow, improve turnaround time (TAT) and simplify inventory management. The kits are stable at room temperature, even when opened, and easy to transfer between laboratories. The first Duraclone standardized dry, pre-formulated panels were introduced in 2014, to support research into the human immune system. Since then, the company has added DuraClone IM and IF panels for investigating immune profiles and function. While preserving strict assay conditions, which is integral to rare event analysis, the DuraClone RE* kits, produced in collaboration with experts from leading clinical research laboratories, enable streamlined workflow and standardization of results that are essential to the experimental robustness of longitudinal and multi-centric research into blood disorders. Internationally-recognized German clinical research scientists commented that the inclusion in the DuraClone RE CLB panels of ROR-1 as a new discriminative marker has markedly facilitated the screening for low frequencies of abnormal B cells and also that the DuraClone Panels provide a sensitive, analytically reliable and economic tool for the detection of minute, abnormal, plasma cell populations. DuraClone RE antibody panels are available in a convenient 25-test package containing three complete compensation kits specific for each panel. They offer the flexibility of additional antibody drop-in slots, making it possible to answer a customer’s specific questions. Further additions to the DuraClone product family are in development, including dry stimulation cocktails for functional assays.