Scientists discover new mechanism that preserves genomic integrity and is abnormal in the rare DiGeorge syndrome

An international team of scientists—including researchers at GENYO, the Centre for Genomics and Oncological Research (Pfizer-University of Granada- Andalusian Regional Government)—has described a molecular mechanism that facilitates the defence of the human genome against ‘bombarding’ by mobile DNA sequences. Abnormalities in the mechanism could be responsible for some symptoms of DiGeorge syndrome, a rare disease. The research could in the future help develop new therapies against the disease, which is caused by the microdeletion of a small part of chromosome 22.

The study describes a sophisticated mechanism that enables all of our cells to control the uncontrolled movement of mobile DNA in our genomes. In patients with DiGeorge syndrome, the cells present abnormalities in the control mechanism. Currently, the research team are trying to generate stem cells that ‘suffer’ from the disease from cells donated by patients who have it—which would enable them to clarify the molecular base of this complex pathology.

DiGeorge syndrome, also known as deletion 22q11.2, is the most common genetic disease caused by a chromosome microdeletion in humans. It has an estimated prevalence of 1 in 4000 births and symptoms vary greatly. Typically, these affect the heart and immune system, as well as presenting as learning difficulties, mental retardation and psychiatric disorders.

The disease is characterised by absence of the ‘Microprocessor’ protein complex, which means these patients lack a ‘vigilante’ gene to watch out for repeated sequences and, therefore, are potentially susceptible to being bombarded by these DNA fragments.
Sara R. Heras—co-author of the study and GENYO researcher—explains that all our cells contain ‘Microprocessor’, a protein complex whose known function at the moment is that of generating small regulatory molecules of ribonucleic acid (RNA), known as microRNAs. ‘Our study has shown that this complex also acts as ‘vigilante’ and defends the integrity of the human genome. Hence, these proteins are capable of recognising and fragmenting the repeated DNA sequences that escape previous control mechanisms, thus preventing them from replicating and introducing themselves into the genome’. University of Granada